Manipulating the Mosquito Microbiota to Study Its Function - ANR - Agence nationale de la recherche Accéder directement au contenu
Chapitre D'ouvrage Année : 2020

Manipulating the Mosquito Microbiota to Study Its Function

Résumé

Aedes aegypti mosquitoes are the main vectors of several arboviruses and are commonly used as models in mosquito biology and vector competence studies. The mosquito microbiota has an impact on different aspects of host physiology, including development, immunity, and fecundity, in turn influencing the capability of the mosquito to transmit diseases. The composition of the microbiota is relatively simple in field mosquitoes, and many of its bacterial members are culturable in the laboratory. Being able to manipulate the composition of the mosquito microbiota is essential to effectively investigate its effect on host physiology and vector competence. This protocol describes how to obtain gnotobiotic mosquitoes, i.e., mosquitoes with a known microbiota composition, and how to monitor the effect of a manipulated microbiota on mosquito development.
Fichier principal
Vignette du fichier
Romoli_Gendrin_book_chapter_revised_with acknowledgements.pdf (369.76 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

pasteur-02919231 , version 1 (21-08-2020)
pasteur-02919231 , version 2 (19-05-2022)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Ottavia Romoli, Mathilde Gendrin. Manipulating the Mosquito Microbiota to Study Its Function. Immunity in Insects, Springer Nature, pp.179-189, 2020, ⟨10.1007/978-1-0716-0259-1_11⟩. ⟨pasteur-02919231v1⟩
68 Consultations
258 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More